Weak base pairing in both seed and 3' regions reduces RNAi off-targets and enhances si/shRNA designs (Q34510032)

2 August 2017

Weak base pairing in both seed and 3' regions reduces RNAi off-targets and enhances si/shRNA designs (English)

1 reference

2 August 2017

0 references

Martin Kampmann

7

0 references

author name string

Shuo Gu

1

1 reference

2 August 2017

Yue Zhang

2

1 reference

2 August 2017

Lan Jin

3

1 reference

2 August 2017

Yong Huang

4

1 reference

2 August 2017

Feijie Zhang

5

1 reference

2 August 2017

Michael C Bassik

6

1 reference

2 August 2017

Mark A Kay

8

1 reference

2 August 2017

language of work or name

English

0 references

publication date

30 September 2014

1 reference

2 August 2017

Nucleic Acids Research

1 reference

2 August 2017

42

1 reference

2 August 2017

12169-12176

1 reference

2 August 2017

19

1 reference

Creative Commons Attribution 4.0 International

2 August 2017

start time

3 May 2017

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

0 references

cites work

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A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility

7 July 2018

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Argonaute divides its RNA guide into domains with distinct functions and RNA-binding properties

7 July 2018

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The loop position of shRNAs and pre-miRNAs is critical for the accuracy of dicer processing in vivo

7 July 2018

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A dynamic perspective of RNAi library development.

7 July 2018

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Rational design of therapeutic siRNAs: minimizing off-targeting potential to improve the safety of RNAi therapy for Huntington's disease.

7 July 2018

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Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs

7 July 2018

1 reference

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RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries

7 July 2018

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Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2

7 July 2018

1 reference

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Functional identification of optimized RNAi triggers using a massively parallel sensor assay

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Mammalian microRNAs predominantly act to decrease target mRNA levels

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Argonaute proteins are key determinants of RNAi efficacy, toxicity, and persistence in the adult mouse liver

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

How do miRNAs mediate translational repression?

7 July 2018

1 reference

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Biochemical principles of small RNA pathways

7 July 2018

1 reference

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RNAi-based therapeutics-current status, challenges and prospects

7 July 2018

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Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application

7 July 2018

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siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect

7 July 2018

1 reference

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Biological basis for restriction of microRNA targets to the 3' untranslated region in mammalian mRNAs

7 July 2018

1 reference

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Biogenesis of small RNAs in animals

7 July 2018

1 reference

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The promises and pitfalls of RNA-interference-based therapeutics

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Thermodynamic stability and Watson-Crick base pairing in the seed duplex are major determinants of the efficiency of the siRNA-based off-target effect.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Modifications in small interfering RNA that separate immunostimulation from RNA interference

7 July 2018

1 reference

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Molecular basis for target RNA recognition and cleavage by human RISC.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

MicroRNA targeting specificity in mammals: determinants beyond seed pairing

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

3' UTR seed matches, but not overall identity, are associated with RNAi off-targets

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Second-generation shRNA libraries covering the mouse and human genomes

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Argonaute2 is the catalytic engine of mammalian RNAi

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

MicroRNAs: genomics, biogenesis, mechanism, and function

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Prediction of mammalian microRNA targets

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Functional siRNAs and miRNAs Exhibit Strand Bias

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Asymmetry in the assembly of the RNAi enzyme complex

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Expression profiling reveals off-target gene regulation by RNAi

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

siRNAs can function as miRNAs

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Improved free-energy parameters for predictions of RNA duplex stability

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Experimental validation of the importance of seed complement frequency to siRNA specificity

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4231738

Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA

27 October 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/25270879

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1093/NAR/GKU854

1 reference

2 August 2017

1 reference

2 August 2017

1 reference