Off-target Effects in CRISPR/Cas9-mediated Genome Engineering (Q26859390)

Louis Y Tee

2

1 reference

Xiao-Gang Wang

3

1 reference

Qun-Shan Huang

4

1 reference

Shi-Hua Yang

5

1 reference

language of work or name

1 reference

17 November 2015

1 reference

Molecular Therapy. Nucleic acids

PubMed

published in

1 reference

volume

4

1 reference

PubMed

page(s)

e264

1 reference

Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system

PubMed

cites work

1 reference

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21 March 2017

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Genome engineering using the CRISPR-Cas9 system

21 March 2017

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CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes

21 March 2017

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RNA-guided editing of bacterial genomes using CRISPR-Cas systems

21 March 2017

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RNA-guided human genome engineering via Cas9

21 March 2017

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Multiplex genome engineering using CRISPR/Cas systems

21 March 2017

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A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

21 March 2017

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Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly

21 March 2017

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Quantifying on- and off-target genome editing

21 March 2017

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Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

21 March 2017

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Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

21 March 2017

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Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

21 March 2017

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Therapeutic genome editing: prospects and challenges

21 March 2017

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Genome editing. The new frontier of genome engineering with CRISPR-Cas9

21 March 2017

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Development and applications of CRISPR-Cas9 for genome engineering

21 March 2017

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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

21 March 2017

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Genome-wide mutation avalanches induced in diploid yeast cells by a base analog or an APOBEC deaminase

21 March 2017

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Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

21 March 2017

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Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

7 April 2017

1 reference

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Genetic screens in human cells using the CRISPR-Cas9 system

7 April 2017

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Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

7 April 2017

1 reference

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CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

CRISPR RNA-guided activation of endogenous human genes

7 April 2017

1 reference

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DNA targeting specificity of RNA-guided Cas9 nucleases

7 April 2017

1 reference

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High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

7 April 2017

1 reference

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Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

7 April 2017

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DNA methylation: roles in mammalian development

7 April 2017

1 reference

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Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

7 April 2017

1 reference

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CRISPR/Cas, the immune system of bacteria and archaea

7 April 2017

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CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

7 April 2017

1 reference

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Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

28 September 2017

1 reference

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Engineered CRISPR-Cas9 nucleases with altered PAM specificities

28 September 2017

1 reference

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Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing

28 September 2017

1 reference

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Cas9-chromatin binding information enables more accurate CRISPR off-target prediction.

28 September 2017

1 reference

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Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

28 September 2017

1 reference

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A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

28 September 2017

1 reference

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Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

Small molecules enhance CRISPR genome editing in pluripotent stem cells.

28 September 2017

1 reference

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A split-Cas9 architecture for inducible genome editing and transcription modulation

28 September 2017

1 reference

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GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila

28 September 2017

1 reference

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Efficient and allele-specific genome editing of disease loci in human iPSCs

28 September 2017

1 reference

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Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

28 September 2017

1 reference

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Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs

28 September 2017

1 reference

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Improved vectors and genome-wide libraries for CRISPR screening

28 September 2017

1 reference

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Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4877446

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease.

28 September 2017

1 reference

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Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

28 September 2017

1 reference

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Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

28 September 2017

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Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA.

28 September 2017

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Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

28 September 2017

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Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos.

28 September 2017

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DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

28 September 2017

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Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

28 September 2017

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Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

28 September 2017

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High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

28 September 2017

1 reference

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TALEN-based gene correction for epidermolysis bullosa.

28 September 2017

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An unbiased genome-wide analysis of zinc-finger nuclease specificity

28 September 2017

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Short motif sequences determine the targets of the prokaryotic CRISPR defence system

28 September 2017

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Trends in large-scale mouse mutagenesis: from genetics to functional genomics

28 September 2017

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A pre-screening FISH-based method to detect CRISPR/Cas9 off-targets in mouse embryonic stem cells.

28 September 2017

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Genome editing technologies: defining a path to clinic

31 May 2018

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Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors.

31 May 2018

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One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system

31 May 2018

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Genome-wide identification of CRISPR/Cas9 off-targets in human genome

31 May 2018

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One-step generation of knockout pigs by zygote injection of CRISPR/Cas system

31 May 2018

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The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

31 May 2018

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Off-target mutations are rare in Cas9-modified mice.

31 May 2018

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Mapping the precision of genome editing.

28 November 2018

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Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.

28 November 2018

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E-CRISP: fast CRISPR target site identification.

28 November 2018

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Heritable gene targeting in the mouse and rat using a CRISPR-Cas system.

28 November 2018

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Generation of gene-modified mice via Cas9/RNA-mediated gene targeting

28 November 2018

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