V(D)J recombination: modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins (Q24324082)

GOA release 2020-03-11

V(D)J recombination

1 reference

GOA release 2020-03-11

author

Michel C. Nussenzweig

6

object named as

M C Nussenzweig

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author name string

D J Sawchuk

1

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F Weis-Garcia

2

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S Malik

3

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E Besmer

4

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M Bustin

5

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P Cortes

Journal of Experimental Medicine

7

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publication date

2 June 1997

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language of work or name

English

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published in

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volume

185

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issue

11

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page(s)

2025-32

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describes a project that uses

ImageQuant

1 reference

Europe PubMed Central

11 June 2022

https://www.ebi.ac.uk/europepmc/webservices/rest/PMC2196333/fullTextXML

based on heuristic

inferred from PubMed Central ID database lookup

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The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination

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The 12/23 rule is enforced at the cleavage step of V(D)J recombination in vivo

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Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates

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Requirement for Ku80 in growth and immunoglobulin V(D)J recombination

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Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

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Double-strand signal sequence breaks in V(D)J recombination are blunt, 5'-phosphorylated, RAG-dependent, and cell cycle regulated

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